pET28-PKP3(305-797)
(Plasmid
#202599)
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PurposeExpresses ARM domain of plakophilin3
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 202599 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28
- Total vector size (bp) 6800
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameplakophilin3
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Alt namePKP3
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SpeciesH. sapiens (human)
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Insert Size (bp)1542
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MutationDeleted amino acids 1 to 304
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Entrez GenePKP3
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Tag
/ Fusion Protein
- His8 tag with precision protease cleavage site (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28-PKP3(305-797) was a gift from Tina Izard (Addgene plasmid # 202599 ; http://n2t.net/addgene:202599 ; RRID:Addgene_202599) -
For your References section:
Plakophilin-3 Binds the Membrane and Filamentous Actin without Bundling F-Actin. Gupta J, Rangarajan ES, Troyanovsky RB, Indra I, Troyanovsky SM, Izard T. Int J Mol Sci. 2023 May 29;24(11):9458. doi: 10.3390/ijms24119458. 10.3390/ijms24119458 PubMed 37298410