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Addgene

pET28-PKP3(305-797)
(Plasmid #202599)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202599 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET28
  • Total vector size (bp) 6800
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    plakophilin3
  • Alt name
    PKP3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1542
  • Mutation
    Deleted amino acids 1 to 304
  • Entrez Gene
    PKP3
  • Tag / Fusion Protein
    • His8 tag with precision protease cleavage site (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28-PKP3(305-797) was a gift from Tina Izard (Addgene plasmid # 202599 ; http://n2t.net/addgene:202599 ; RRID:Addgene_202599)
  • For your References section:

    Plakophilin-3 Binds the Membrane and Filamentous Actin without Bundling F-Actin. Gupta J, Rangarajan ES, Troyanovsky RB, Indra I, Troyanovsky SM, Izard T. Int J Mol Sci. 2023 May 29;24(11):9458. doi: 10.3390/ijms24119458. 10.3390/ijms24119458 PubMed 37298410