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vYM245_PB-Blast-pEF1a-H2B-mCh-D3bCI
(Plasmid #202596)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202596 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PiggyBac
  • Backbone size w/o insert (bp) 6300
  • Total vector size (bp) 10800
  • Vector type
    Mammalian Expression, Synthetic Biology
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H2B-mCherry-T2A-rTetR-DNMT3bCI
  • Species
    M. musculus (mouse), Synthetic
  • Insert Size (bp)
    4500
  • Mutation
    P656V, C657D
  • GenBank ID
    NM_001003961
  • Entrez Gene
    Dnmt3b (a.k.a. MmuIIIB)
  • Tag / Fusion Protein
    • rTetR (N terminal on insert)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.05.30.542205 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    vYM245_PB-Blast-pEF1a-H2B-mCh-D3bCI was a gift from Michael Elowitz (Addgene plasmid # 202596 ; http://n2t.net/addgene:202596 ; RRID:Addgene_202596)

  • For your References section:

    Tuning Methylation-Dependent Silencing Dynamics by Synthetic Modulation of CpG Density. Ma Y, Budde MW, Zhu J, Elowitz MB. ACS Synth Biol. 2023 Aug 12. doi: 10.1021/acssynbio.3c00078. 10.1021/acssynbio.3c00078 PubMed 37572041
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