CamKIIa:smFP-TVA-RGV
(Plasmid
#202594)
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PurposeExpresses TVA receptor, rabies oG and “spaghetti monster” fluorescent protein in mature excitatory neurons.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 202594 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAddgene plasmid #96941
- Backbone size w/o insert (bp) 9199
- Total vector size (bp) 12452
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert namesmFP
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Alt namespaghetti monster fluorescent protein
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Insert Size (bp)843
Gene/Insert 2
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Gene/Insert nameTVA
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Alt nameavian receptor protein
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Insert Size (bp)471
Gene/Insert 3
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Gene/Insert nameRGV
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Alt namerabies glycoprotein (G)
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Insert Size (bp)1572
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CamKIIa:smFP-TVA-RGV was a gift from Oleksandr Shcheglovitov (Addgene plasmid # 202594 ; http://n2t.net/addgene:202594 ; RRID:Addgene_202594) -
For your References section:
Defective AMPA-mediated synaptic transmission and morphology in human neurons with hemizygous SHANK3 deletion engrafted in mouse prefrontal cortex. Chiola S, Napan KL, Wang Y, Lazarenko RM, Armstrong CJ, Cui J, Shcheglovitov A. Mol Psychiatry. 2021 Feb 8. pii: 10.1038/s41380-021-01023-2. doi: 10.1038/s41380-021-01023-2. 10.1038/s41380-021-01023-2 PubMed 33558651