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pRSF1b-His8-TEV-PI31-1-151
(Plasmid #202593)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202593 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSF1b
  • Total vector size (bp) 4068
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PI31
  • Species
    H. sapiens (human)
  • Mutation
    aa 1-151 only
  • Entrez Gene
    PSMF1 (a.k.a. PI31)
  • Tag / Fusion Protein
    • 8xHis, TEV (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2022.11.02.514817 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSF1b-His8-TEV-PI31-1-151 was a gift from Brenda Schulman (Addgene plasmid # 202593 ; http://n2t.net/addgene:202593 ; RRID:Addgene_202593)

  • For your References section:

    PARK15/FBXO7 is dispensable for PINK1/Parkin mitophagy in iNeurons and HeLa cell systems. Kraus F, Goodall EA, Smith IR, Jiang Y, Paoli JC, Adolf F, Zhang J, Paulo JA, Schulman BA, Harper JW. EMBO Rep. 2023 Aug 3;24(8):e56399. doi: 10.15252/embr.202256399. Epub 2023 Jun 19. 10.15252/embr.202256399 PubMed 37334901
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