pRSF1b-His8-TEV-PI31-1-151
(Plasmid
#202593)
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PurposeBacterial expression of HsPI31-1-151
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 202593 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRSF1b
- Total vector size (bp) 4068
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namePI31
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SpeciesH. sapiens (human)
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Mutationaa 1-151 only
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Entrez GenePSMF1 (a.k.a. PI31)
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Tag
/ Fusion Protein
- 8xHis, TEV (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2022.11.02.514817 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRSF1b-His8-TEV-PI31-1-151 was a gift from Brenda Schulman (Addgene plasmid # 202593 ; http://n2t.net/addgene:202593 ; RRID:Addgene_202593) -
For your References section:
PARK15/FBXO7 is dispensable for PINK1/Parkin mitophagy in iNeurons and HeLa cell systems. Kraus F, Goodall EA, Smith IR, Jiang Y, Paoli JC, Adolf F, Zhang J, Paulo JA, Schulman BA, Harper JW. EMBO Rep. 2023 Aug 3;24(8):e56399. doi: 10.15252/embr.202256399. Epub 2023 Jun 19. 10.15252/embr.202256399 PubMed 37334901
