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pGEX4T1-TEV-HsFbxo7-129-398/Skp1
(Plasmid #202592)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202592 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX4T1
  • Total vector size (bp) 6350
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    Fbxo7
  • Species
    H. sapiens (human)
  • Mutation
    aa 129-398 only
  • Entrez Gene
    FBXO7 (a.k.a. FBX, FBX07, FBX7, PARK15, PKPS)
  • Tag / Fusion Protein
    • GST, TEV (N terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    Skp1
  • Species
    H. sapiens (human)
  • Entrez Gene
    SKP1 (a.k.a. EMC19, OCP-II, OCP2, SKP1A, TCEB1L, p19A)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2022.11.02.514817 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX4T1-TEV-HsFbxo7-129-398/Skp1 was a gift from Brenda Schulman (Addgene plasmid # 202592 ; http://n2t.net/addgene:202592 ; RRID:Addgene_202592)

  • For your References section:

    PARK15/FBXO7 is dispensable for PINK1/Parkin mitophagy in iNeurons and HeLa cell systems. Kraus F, Goodall EA, Smith IR, Jiang Y, Paoli JC, Adolf F, Zhang J, Paulo JA, Schulman BA, Harper JW. EMBO Rep. 2023 Aug 3;24(8):e56399. doi: 10.15252/embr.202256399. Epub 2023 Jun 19. 10.15252/embr.202256399 PubMed 37334901
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