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pETM11-6xHis-TEV-hORF1p_R261A
(Plasmid #202586)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202586 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pETM11
  • Backbone size w/o insert (bp) 5300
  • Total vector size (bp) 6369
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ORF1p
  • Alt name
    ORF1
  • Alt name
    L1ORF1p
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1090
  • Mutation
    Changed ORF1p Arginine 261 to Alanine
  • Tags / Fusion Proteins
    • 6x His (N terminal on insert)
    • TEV protease cleavage site (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETM11-6xHis-TEV-hORF1p_R261A was a gift from Liam Holt (Addgene plasmid # 202586 ; http://n2t.net/addgene:202586 ; RRID:Addgene_202586)

  • For your References section:

    Condensation of LINE-1 is critical for retrotransposition. Sil S, Keegan S, Ettefa F, Denes L, Boeke JD, Holt LJ. Elife. 2023 Apr 28;12:e82991. doi: 10.7554/eLife.82991. 10.7554/eLife.82991 PubMed 37114770
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