pZG61
(Plasmid
#202575)
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PurposeRNA editing in plants. Plasmid contains 35S-dADARcd-LIC1-ccdB-LIC2-35S-hptII
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 202575 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepJG054
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHyperADARcd
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Alt nameadenosine deaminase acting on RNA (ADAR) catalytic domain
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SpeciesD. melanogaster (fly)
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MutationE488Q
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Genbank: MW026677
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pZG61 was a gift from Guoyong Xu (Addgene plasmid # 202575 ; http://n2t.net/addgene:202575 ; RRID:Addgene_202575) -
For your References section:
Proximity editing to identify RNAs in phase-separated RNA binding protein condensates. Zhou G, Niu R, Zhou Y, Luo M, Peng Y, Wang H, Wang Z, Xu G. Cell Discov. 2021 Aug 24;7(1):72. doi: 10.1038/s41421-021-00288-9. 10.1038/s41421-021-00288-9 PubMed 34426573