pCEP-Puro-TetOn-L1RP-ORF1_StammerAAA_Halo-GFPai
(Plasmid #202571)

• Purpose: Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the M91A, E92A, and L93A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR
• Depositing Lab: Liam Holt
• Publication: Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.7554/eLife.82991.

Backbone

• Vector backbone: pCEP-Puro
• Backbone size w/o insert (bp): 10000
• Total vector size (bp): 19319
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: LINE-1
• Alt name: L1RP
• Alt name: L1
• Species: H. sapiens (human)
• Insert Size (bp): 9613
• Mutation: Changed ORF1p Methionine 91 to Alanine, ORF1p Glutamic Acid 92 to Alanine, ORF1p Leucine 93 to Alanine
• Tags / Fusion Proteins:
  • HaloTag7 on ORF1p (C terminal on insert)
  • GFP-AI retrotransposition reporter (C terminal on insert)

Resource Information

• Supplemental Documents:
  • pLH2041_SS0022.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry