pCEP-Puro-TetOn-L1RP-ORF1_R261A_Halo-GFPai
(Plasmid
#202569)
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PurposeExpresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the R261A mutation and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 202569 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCEP-Puro
- Backbone size w/o insert (bp) 10000
- Total vector size (bp) 19319
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLINE-1
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Alt nameL1RP
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Alt nameL1
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SpeciesH. sapiens (human)
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Insert Size (bp)9613
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MutationChanged ORF1p Arginine 261 to Alanine
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Tags
/ Fusion Proteins
- HaloTag7 on ORF1p (C terminal on insert)
- GFP-AI retrotransposition reporter (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCEP-Puro-TetOn-L1RP-ORF1_R261A_Halo-GFPai was a gift from Liam Holt (Addgene plasmid # 202569 ; http://n2t.net/addgene:202569 ; RRID:Addgene_202569) -
For your References section:
Condensation of LINE-1 is critical for retrotransposition. Sil S, Keegan S, Ettefa F, Denes L, Boeke JD, Holt LJ. Elife. 2023 Apr 28;12:e82991. doi: 10.7554/eLife.82991. 10.7554/eLife.82991 PubMed 37114770