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Addgene

pCEP-Puro-TetOn-L1RP-ORF1_R261A_Halo-GFPai
(Plasmid #202569)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202569 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCEP-Puro
  • Backbone size w/o insert (bp) 10000
  • Total vector size (bp) 19319
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LINE-1
  • Alt name
    L1RP
  • Alt name
    L1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    9613
  • Mutation
    Changed ORF1p Arginine 261 to Alanine
  • Tags / Fusion Proteins
    • HaloTag7 on ORF1p (C terminal on insert)
    • GFP-AI retrotransposition reporter (C terminal on insert)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCEP-Puro-TetOn-L1RP-ORF1_R261A_Halo-GFPai was a gift from Liam Holt (Addgene plasmid # 202569 ; http://n2t.net/addgene:202569 ; RRID:Addgene_202569)
  • For your References section:

    Condensation of LINE-1 is critical for retrotransposition. Sil S, Keegan S, Ettefa F, Denes L, Boeke JD, Holt LJ. Elife. 2023 Apr 28;12:e82991. doi: 10.7554/eLife.82991. 10.7554/eLife.82991 PubMed 37114770