pCEP-Puro-TetOn-L1RP-ORF1_mNeonGreen2
(Plasmid #202566)

• Purpose: Expresses an endogenous-like L1RP LINE-1 element with a C-terminal mNeonGreen2 fluorophore on the ORF1 protein for fluorescence microscopy
• Depositing Lab: Liam Holt
• Publication: Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.7554/eLife.82991.

Backbone

• Vector backbone: pCEP-Puro
• Backbone size w/o insert (bp): 9800
• Total vector size (bp): 16623
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: LINE-1
• Alt name: L1RP
• Alt name: L1
• Species: H. sapiens (human)
• Insert Size (bp): 6854
• Tag / Fusion Protein:
  • mNeonGreen2 on ORF1p (C terminal on insert)

Resource Information

• Supplemental Documents:
  • pLH2060_SS0043.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry