LentiGuide_CjCas9-Puro
(Plasmid
#202564)
-
PurposehU6-BsmBI-gRNA-Puro
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 202564 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonelentiGuide-Puro
-
Vector typeMammalian Expression
-
Selectable markersBlasticidin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCjCas9 sgRNA cassette
-
Alt nameCampylobacter jejuni CRISPR customizable RNA element
-
gRNA/shRNA sequencecustomizable
-
SpeciesSynthetic
- Promoter hU6
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsmBI (unknown if destroyed)
- 3′ cloning site BsmBI (unknown if destroyed)
- 5′ sequencing primer hU6-F (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
LentiGuide_CjCas9-Puro was a gift from Gerald Schwank (Addgene plasmid # 202564 ; http://n2t.net/addgene:202564 ; RRID:Addgene_202564) -
For your References section:
Continuous directed evolution of a compact CjCas9 variant with broad PAM compatibility. Schmidheini L, Mathis N, Marquart KF, Rothgangl T, Kissling L, Bock D, Chanez C, Wang JP, Jinek M, Schwank G. Nat Chem Biol. 2023 Sep 21. doi: 10.1038/s41589-023-01427-x. 10.1038/s41589-023-01427-x PubMed 37735239