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Addgene

mycUb-25aa
(Plasmid #20256)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20256 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-Myc
  • Backbone size w/o insert (bp) 3300
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ubiquitin-25 amino acid extension
  • Alt name
    Ub-25aa
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4063
  • Mutation
    Ubiquitin fused to N-terminus of GFP. Glycine 76 mutated to Valine. Stop codon introduced after 25 amino acids in GFP
  • Entrez Gene
    RBX1 (a.k.a. BA554C12.1, RNF75, ROC1)
  • Tag / Fusion Protein
    • myc (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mycUb-25aa was a gift from Nico Dantuma (Addgene plasmid # 20256 ; http://n2t.net/addgene:20256 ; RRID:Addgene_20256)

  • For your References section:

    Minimal length requirement for proteasomal degradation of ubiquitin-dependent substrates. Verhoef LG, Heinen C, Selivanova A, Halff EF, Salomons FA, Dantuma NP. FASEB J. 2009 Jan . 23(1):123-33. 10.1096/fj.08-115055 PubMed 18796559
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