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Addgene

pCMV-3UTR
(Plasmid #202550)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202550 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-Script
  • Backbone manufacturer
    Agilent Technologies
  • Backbone size (bp) 4363
  • Modifications to backbone
    Original MCS is replaced by an ATG initiation codon, signaling peptide and a new cloning cassette. EF1 alpha 3'UTR region included as well.
  • Vector type
    Mammalian Expression
  • Promoter CMV
  • Selectable markers
    Neomycin (select with G418)
  • Tags / Fusion Proteins
    • ATG initiation codon followed by an IGKV3 signal peptide and AgeI site for seamless cloning of the gene of interest (N terminal on backbone)
    • none (C terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-3UTR was a gift from Rostislav Skrabana (Addgene plasmid # 202550 ; http://n2t.net/addgene:202550 ; RRID:Addgene_202550)

  • For your References section:

    Cost-Effective Protein Production in CHO Cells Following Polyethylenimine-Mediated Gene Delivery Showcased by the Production and Crystallization of Antibody Fabs. Meskova K, Martonova K, Hrasnova P, Sinska K, Skrabanova M, Fialova L, Njemoga S, Cehlar O, Parmar O, Kolenko P, Pevala V, Skrabana R. Antibodies (Basel). 2023 Aug 4;12(3):51. doi: 10.3390/antib12030051. 10.3390/antib12030051 PubMed 37606435
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