pCMV-3UTR
(Plasmid
#202550)
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Purpose(Empty Backbone) Ready for cloning and heterologous expression of genes in mammalian cells with protein export to cultivation media
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 202550 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV-Script
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Backbone manufacturerAgilent Technologies
- Backbone size (bp) 4363
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Modifications to backboneOriginal MCS is replaced by an ATG initiation codon, signaling peptide and a new cloning cassette. EF1 alpha 3'UTR region included as well.
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Vector typeMammalian Expression
- Promoter CMV
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Selectable markersNeomycin (select with G418)
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Tags
/ Fusion Proteins
- ATG initiation codon followed by an IGKV3 signal peptide and AgeI site for seamless cloning of the gene of interest (N terminal on backbone)
- none (C terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-3UTR was a gift from Rostislav Skrabana (Addgene plasmid # 202550 ; http://n2t.net/addgene:202550 ; RRID:Addgene_202550) -
For your References section:
Cost-Effective Protein Production in CHO Cells Following Polyethylenimine-Mediated Gene Delivery Showcased by the Production and Crystallization of Antibody Fabs. Meskova K, Martonova K, Hrasnova P, Sinska K, Skrabanova M, Fialova L, Njemoga S, Cehlar O, Parmar O, Kolenko P, Pevala V, Skrabana R. Antibodies (Basel). 2023 Aug 4;12(3):51. doi: 10.3390/antib12030051. 10.3390/antib12030051 PubMed 37606435
Map uploaded by the depositor.
