mycUBB+1
(Plasmid
#20254)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 20254 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV-Myc
- Backbone size w/o insert (bp) 3300
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUbiquitin B +1 transcript
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Alt nameUbb+1
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SpeciesH. sapiens (human)
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Insert Size (bp)4063
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Entrez GeneUBB (a.k.a. HEL-S-50)
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Tag
/ Fusion Protein
- myc (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mycUBB+1 was a gift from Nico Dantuma (Addgene plasmid # 20254 ; http://n2t.net/addgene:20254 ; RRID:Addgene_20254) -
For your References section:
Minimal length requirement for proteasomal degradation of ubiquitin-dependent substrates. Verhoef LG, Heinen C, Selivanova A, Halff EF, Salomons FA, Dantuma NP. FASEB J. 2009 Jan . 23(1):123-33. 10.1096/fj.08-115055 PubMed 18796559