Skip to main content
Addgene

Ub[9]-mRFP[1]
(Plasmid #20249)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20249 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    mRFP-N1
  • Backbone size w/o insert (bp) 4678
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ubiquitin C
  • Alt name
    UBC
  • Alt name
    UBC-mRFP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6755
  • Mutation
    changed Stop codon 686 to Glycine
  • GenBank ID
    NM_021009
  • Entrez Gene
    UBC (a.k.a. HMG20)
  • Tag / Fusion Protein
    • mRFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ub[9]-mRFP[1] was a gift from Nico Dantuma (Addgene plasmid # 20249 ; http://n2t.net/addgene:20249 ; RRID:Addgene_20249)

  • For your References section:

    Selective Accumulation of Aggregation-Prone Proteasome Substrates in Response to Proteotoxic Stress. Salomons FA, Menendez-Benito V, Boettcher C, McCray B, Taylor JP, Dantuma NP. Mol Cell Biol. 2009 Jan 21. ():. 10.1128/MCB.01485-08 PubMed 19158272
Related items:
Commonly requested with: