pCOLA-Gent-EM7-Erv1p
(Plasmid
#202484)
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PurposeExpresses sulfhydryl oxidase (Erv1p) constitutively
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 202484 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCOLA
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namesulfydryl oxidase (Erv1p)
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Alt nameErv1p
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SpeciesS. cerevisiae (budding yeast)
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Entrez GeneERV1 (a.k.a. YGR029W)
- Promoter EM7
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CTCAAGGGCATCGGTCGAGA
- 3′ sequencing primer AAGTTGGAACCTCTTACGTGC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCOLA-Gent-EM7-Erv1p was a gift from Michael Lynch (Addgene plasmid # 202484 ; http://n2t.net/addgene:202484 ; RRID:Addgene_202484) -
For your References section:
Scalable, robust, high-throughput expression & purification of nanobodies enabled by 2-stage dynamic control. Hennigan JN, Menacho-Melgar R, Sarkar P, Golovsky M, Lynch MD. Metab Eng. 2024 Sep;85:116-130. doi: 10.1016/j.ymben.2024.07.012. Epub 2024 Jul 24. 10.1016/j.ymben.2024.07.012 PubMed 39059674