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pattB-LSL-AAVR-F2A-spCas9
(Plasmid #202459)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202459 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBT378, Addgene, cat#52554
  • Backbone manufacturer
    Liqun Luo
  • Backbone size w/o insert (bp) 5510
  • Total vector size (bp) 16316
  • Modifications to backbone
    Previously modified by Monte Winslow to express Streptococcus pyogenes Cas9 (spCas9) with a LSL sequence proceeding to generate paatB-LSL-spCas9.
  • Vector type
    Mammalian Expression, Mouse Targeting, Cre/Lox, CRISPR
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AAVR
  • Alt name
    AU040320
  • Alt name
    Kiaa0319L
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3144
  • Entrez Gene
    AU040320 (a.k.a. A730047D20Rik, AAVR, Kiaa0319l)
  • Promoter CAG
  • Tag / Fusion Protein
    • mCherry fusion (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer gtaaaacgacggccagt
  • 3′ sequencing primer gtcatagctgtttcctg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pattB-LSL-AAVR-F2A-spCas9 was a gift from Jan Carette (Addgene plasmid # 202459 ; http://n2t.net/addgene:202459 ; RRID:Addgene_202459)

  • For your References section:

    Hardwiring tissue-specific AAV transduction in mice through engineered receptor expression. Zengel J, Wang YX, Seo JW, Ning K, Hamilton JN, Wu B, Raie M, Holbrook C, Su S, Clements DR, Pillay S, Puschnik AS, Winslow MM, Idoyaga J, Nagamine CM, Sun Y, Mahajan VB, Ferrara KW, Blau HM, Carette JE. Nat Methods. 2023 Jul;20(7):1070-1081. doi: 10.1038/s41592-023-01896-x. Epub 2023 Jun 8. 10.1038/s41592-023-01896-x PubMed 37291262
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