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Addgene

pmNeon Green-N1-ARF1N/3C-R
(Plasmid #202433)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202433 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmNeon Green-N1
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ARF1
  • Species
    H. sapiens (human)
  • Mutation
    C terminus replaced with ARF3 sequence (S174A/R178K/Q180K)
  • Entrez Gene
    ARF1 (a.k.a. PVNH8)
  • Tag / Fusion Protein
    • mNeonGreen (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmNeon Green-N1-ARF1N/3C-R was a gift from David Bryant (Addgene plasmid # 202433 ; http://n2t.net/addgene:202433 ; RRID:Addgene_202433)

  • For your References section:

    The small GTPase ARF3 controls invasion modality and metastasis by regulating N-cadherin levels. Sandilands E, Freckmann EC, Cumming EM, Roman-Fernandez A, McGarry L, Anand J, Galbraith L, Mason S, Patel R, Nixon C, Cartwright J, Leung HY, Blyth K, Bryant DM. J Cell Biol. 2023 Apr 3;222(4):e202206115. doi: 10.1083/jcb.202206115. Epub 2023 Feb 28. 10.1083/jcb.202206115 PubMed 36880595
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