pQCXIH FRB-BFP
(Plasmid
#202425)
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PurposeTagBFP FKBP-rapamycin binding (FRB) dimerization domain
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 202425 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepQCXIZ BFP
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFKBP-rapamycin binding (FRB) dimerization domain
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SpeciesSynthetic
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Tag
/ Fusion Protein
- TagBFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pQCXIH FRB-BFP was a gift from David Bryant (Addgene plasmid # 202425 ; http://n2t.net/addgene:202425 ; RRID:Addgene_202425) -
For your References section:
Spatial regulation of the glycocalyx component podocalyxin is a switch for prometastatic function. Roman-Fernandez A, Mansour MA, Kugeratski FG, Anand J, Sandilands E, Galbraith L, Rakovic K, Freckmann EC, Cumming EM, Park J, Nikolatou K, Lilla S, Shaw R, Strachan D, Mason S, Patel R, McGarry L, Katoch A, Campbell KJ, Nixon C, Miller CJ, Leung HY, Le Quesne J, Norman JC, Zanivan S, Blyth K, Bryant DM. Sci Adv. 2023 Feb 3;9(5):eabq1858. doi: 10.1126/sciadv.abq1858. Epub 2023 Feb 3. 10.1126/sciadv.abq1858 PubMed 36735782