pLenti-U6-DCK-Hygro
(Plasmid #202409)

• Purpose: gRNA expression vector for DCK
• Depositing Lab: Morito Kurata
• Publication: Sugita et al Commun Biol. 2023 Jun 1;6(1):582. doi: 10.1038/s42003-023-04941-9.

Backbone

• Vector backbone: lentiGuide-Puro
• Backbone manufacturer: Addgene
• Backbone size w/o insert (bp): 8322
• Total vector size (bp): 8330
• Vector type: Lentiviral, CRISPR
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: guide RNA
• gRNA/shRNA sequence: AAGTACTCAAGATGAATTTG
• Species: H. sapiens (human)
• Insert Size (bp): 1000
• Entrez Gene: DCK
• Promoter: U6
• Tag / Fusion Protein:
  • Dck

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsmBⅠ (unknown if destroyed)
• 3′ cloning site: BsmBⅠ (unknown if destroyed)
• 5′ sequencing primer: M13

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2022.07.15.500173v1 for bioRxiv preprint.