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Addgene

pJYP1
(Plasmid #202406)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202406 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET22b
  • Modifications to backbone
    The pET22b expression vector was modified by inserting the fdeR transcriptional activator and promoter operator region (PfdeA – fdeO) followed by a vitamin B12 riboswitch (RSB12) into the HpaI and XhoI site. The resulting pJYP1 vector contains a BglII restriction site downstream of the riboswitch to allow toxin cloning.
  • Vector type
    Bacterial Expression
  • Promoter fdeA

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJYP1 was a gift from Remy Loris (Addgene plasmid # 202406 ; http://n2t.net/addgene:202406 ; RRID:Addgene_202406)

  • For your References section:

    A Multi-Layer-Controlled Strategy for Cloning and Expression of Toxin Genes in Escherichia coli. Vandierendonck J, Girardin Y, De Bruyn P, De Greve H, Loris R. Toxins (Basel). 2023 Aug 18;15(8):508. doi: 10.3390/toxins15080508. 10.3390/toxins15080508 PubMed 37624265
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