pE1B-C-F/Sox10-MCS1B
(Plasmid
#20239)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 20239 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepE1B-C-F
- Backbone size w/o insert (bp) 4909
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSox10-MCS1B
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SpeciesM. musculus (mouse)
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Insert Size (bp)723
Cloning Information
- Cloning method Gateway Cloning
- 5′ cloning site attR (destroyed during cloning)
- 3′ cloning site attR (destroyed during cloning)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pE1B-C-F/Sox10-MCS1B was a gift from William Pavan (Addgene plasmid # 20239 ; http://n2t.net/addgene:20239 ; RRID:Addgene_20239) -
For your References section:
Identification of neural crest and glial enhancers at the mouse Sox10 locus through transgenesis in zebrafish. Antonellis A, Huynh JL, Lee-Lin SQ, Vinton RM, Renaud G, Loftus SK, Elliot G, Wolfsberg TG, Green ED, McCallion AS, Pavan WJ. PLoS Genet. 2008 . 4(9):e1000174. 10.1371/journal.pgen.1000174 PubMed 18773071