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pRmHa3-dKDM4A-H195A-HA2FL2
(Plasmid #20235)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20235 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRmHa3-CHA2FL2
  • Backbone size w/o insert (bp) 3990
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dKDM4A
  • Alt name
    CG15835
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    1488
  • Mutation
    changed His 195 to Ala
  • GenBank ID
    NM_136487
  • Entrez Gene
    Kdm4A (a.k.a. CG15835, dJMJD2(1), dKDM4A, Dmel\CG15835)
  • Tags / Fusion Proteins
    • HA2 (C terminal on backbone)
    • FLAG2 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer agaggtgaatcgaacgaaagacccg
  • 3′ sequencing primer gtagagcgtttatcagttttttgg
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

D153E mutation is also present, but it does affect the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRmHa3-dKDM4A-H195A-HA2FL2 was a gift from Jerry Workman (Addgene plasmid # 20235 ; http://n2t.net/addgene:20235 ; RRID:Addgene_20235)

  • For your References section:

    Heterochromatin protein 1a stimulates histone H3 lysine 36 demethylation by the Drosophila KDM4A demethylase. Lin CH, Li B, Swanson S, Zhang Y, Florens L, Washburn MP, Abmayr SM, Workman JL. Mol Cell. 2008 Dec 5. 32(5):696-706. 10.1016/j.molcel.2008.11.008 PubMed 19061644
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