pAJT194
(Plasmid
#202293)
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PurposeType IIS cloning plasmid for LPattB5 integrations w/ OriT
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 202293 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneR6K
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Pir1
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namesfGFP
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAJT194 was a gift from Christopher Voigt (Addgene plasmid # 202293 ; http://n2t.net/addgene:202293 ; RRID:Addgene_202293) -
For your References section:
Redesign of an Escherichia coli Nissle treatment for phenylketonuria using insulated genomic landing pads and genetic circuits to reduce burden. Triassi AJ, Fields BD, Monahan CE, Means JM, Park Y, Doosthosseini H, Padmakumar JP, Isabella VM, Voigt CA. Cell Syst. 2023 Jun 21;14(6):512-524.e12. doi: 10.1016/j.cels.2023.05.004. 10.1016/j.cels.2023.05.004 PubMed 37348465