hTRAF2pLPCX-HA-Flag/P874
(Plasmid #20229)

• Depositing Lab: Martin Dorf
• Publication: Li et al Mol Cell. 2009 Jan 16. 33(1):30-42.

Backbone

• Vector backbone: pLPCX
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 6203
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: TNF receptor-associated factor 2
• Alt name: TRAF2
• Alt name: TRAP
• Alt name: TRAP3
• Species: H. sapiens (human)
• Insert Size (bp): 1567
• Mutation: unmutated NM_021138.3. L replaces M at beginning of CDS after HA epitope.
• GenBank ID: NM_021138
• Entrez Gene: TRAF2 (a.k.a. MGC:45012, RNF117, TRAP, TRAP3)
• Tags / Fusion Proteins:
  • HA (N terminal on insert)
  • FLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (destroyed during cloning)
• 3′ cloning site: ClaI (not destroyed)
• 5′ sequencing primer: pLNCX5' or CMV primer
• 3′ sequencing primer: pLNCX3'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: ATTC
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry