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Addgene

pBC115
(Plasmid #202277)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202277 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBCC091
  • Vector type
    Bacterial Expression
  • Selectable markers
    Tetracyline

Growth in Bacteria

  • Bacterial Resistance(s)
    Kan + DAP
  • Growth Temperature
    37°C
  • Growth Strain(s)
    E.coli BW29427
  • Growth instructions
    Requires media that contains DAP. A supplement
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    bc142
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid can be transformed into E. coli BW29427 supplemented with 50 µg/mL of diaminopimelic acid (DAP) or any Pir1 strain. Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBC115 was a gift from Paul Schulze-Lefert (Addgene plasmid # 202277 ; http://n2t.net/addgene:202277 ; RRID:Addgene_202277)

  • For your References section:

    Chromosomal barcodes for simultaneous tracking of near-isogenic bacterial strains in plant microbiota. Ordon J, Thouin J, Nakano RT, Ma KW, Zhang P, Huettel B, Garrido-Oter R, Schulze-Lefert P. Nat Microbiol. 2024 Apr;9(4):1117-1129. doi: 10.1038/s41564-024-01619-8. Epub 2024 Mar 19. 10.1038/s41564-024-01619-8 PubMed 38503974
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