3xFRB-myc
(Plasmid #20226)

• Depositing Lab: Carolyn Bertozzi
• Publication: Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186-7.

Backbone

• Vector backbone: pcDNA3.1/Zeo(+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5000
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 3xFRB
• Alt name: 3 copies of fused FRB
• Alt name: mTOR domain
• Species: H. sapiens (human)
• Insert Size (bp): 849
• Mutation: 1 FRB copy has a T2098L point mutation
• Entrez Gene: MTOR (a.k.a. FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS)
• Tag / Fusion Protein:
  • myc (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: AvrII (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry