pBCC091
(Plasmid
#202218)
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PurposeLevel 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Tetracyline selectable marker and far-red-FP fluorescent marker mPlum
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 202218 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBCC34
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Vector typeBacterial Expression
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Selectable markersTetracyline
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Kanamycin, 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DB3.1λpir
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namepBCC25, pBCC30, pBCC55, pBCC27, pBCC26, pBCC28, pICH41822 (G5)
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBCC091 was a gift from Paul Schulze-Lefert (Addgene plasmid # 202218 ; http://n2t.net/addgene:202218 ; RRID:Addgene_202218) -
For your References section:
Chromosomal barcodes for simultaneous tracking of near-isogenic bacterial strains in plant microbiota. Ordon J, Thouin J, Nakano RT, Ma KW, Zhang P, Huettel B, Garrido-Oter R, Schulze-Lefert P. Nat Microbiol. 2024 Apr;9(4):1117-1129. doi: 10.1038/s41564-024-01619-8. Epub 2024 Mar 19. 10.1038/s41564-024-01619-8 PubMed 38503974