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Addgene

pBCC089
(Plasmid #202216)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202216 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBCC34
  • Vector type
    Bacterial Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin, 25 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1λpir
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    pBCC25, pBCC33, pBCC59, pBCC27, pBCC26, pBCC28, pICH41822 (G5)
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBCC089 was a gift from Paul Schulze-Lefert (Addgene plasmid # 202216 ; http://n2t.net/addgene:202216 ; RRID:Addgene_202216)

  • For your References section:

    Chromosomal barcodes for simultaneous tracking of near-isogenic bacterial strains in plant microbiota. Ordon J, Thouin J, Nakano RT, Ma KW, Zhang P, Huettel B, Garrido-Oter R, Schulze-Lefert P. Nat Microbiol. 2024 Apr;9(4):1117-1129. doi: 10.1038/s41564-024-01619-8. Epub 2024 Mar 19. 10.1038/s41564-024-01619-8 PubMed 38503974
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