pBCC077
(Plasmid
#202211)
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PurposeLevel1 DNA-Tag with bc065
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 202211 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBCC27
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namebc065
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBCC077 was a gift from Paul Schulze-Lefert (Addgene plasmid # 202211 ; http://n2t.net/addgene:202211 ; RRID:Addgene_202211) -
For your References section:
Chromosomal barcodes for simultaneous tracking of near-isogenic bacterial strains in plant microbiota. Ordon J, Thouin J, Nakano RT, Ma KW, Zhang P, Huettel B, Garrido-Oter R, Schulze-Lefert P. Nat Microbiol. 2024 Apr;9(4):1117-1129. doi: 10.1038/s41564-024-01619-8. Epub 2024 Mar 19. 10.1038/s41564-024-01619-8 PubMed 38503974