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pBIG2abc_hsHAUScomplex_FL
(Plasmid #202203)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202203 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBIG2abc
  • Backbone size w/o insert (bp) 5669
  • Vector type
    Insect Expression
  • Selectable markers
    Tetracycline

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The depositor uses DH10EmBacY as the growing strain for the plasmid while transferring the plasmid to the Baculovirus system.
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    HAUS1
  • Species
    H. sapiens (human); codon-optimized form for expression in Trichoplusia ni cells
  • GenBank ID
    NM_138443.4
  • Entrez Gene
    HAUS1 (a.k.a. CCDC5, HEI-C, HEIC, HsT1461)
  • Promoter polyhedrin promoter
  • Tag / Fusion Protein
    • His-tag (6x) (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer aacgctctatggtctaaag
  • 3′ sequencing primer aaacgtgcaatagtatc
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    HAUS3
  • Species
    H. sapiens (human); codon-optimized form for expression in Trichoplusia ni cells
  • GenBank ID
    NM_001303143.2
  • Entrez Gene
    HAUS3 (a.k.a. IT1, dgt3, C4orf15, MGC4701, DKFZp686I1868)
  • Promoter polyhedrin promoter

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer AAACTGGATACTATTGCAC
  • 3′ sequencing primer aaccccgattgagatatag
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    HAUS2
  • Species
    H. sapiens (human); codon-optimized form for expression in Trichoplusia ni cells
  • GenBank ID
    NM_018097.3
  • Entrez Gene
    HAUS2 (a.k.a. CEP27, C15orf25, FLJ10460, HsT17025)
  • Promoter polyhedrin promoter
  • Tag / Fusion Protein
    • -mGFP-TEV-TwinStrep (C terminal on insert)

Cloning Information for Gene/Insert 3

  • Cloning method Gibson Cloning
  • 5′ sequencing primer aacgctctatggtctaaag
  • 3′ sequencing primer aaacgtgcaatagtatc
    • (Common Sequencing Primers)

Gene/Insert 4

  • Gene/Insert name
    HAUS6
  • Species
    H. sapiens (human); codon-optimized form for expression in Trichoplusia ni cells
  • GenBank ID
    NM_017645.5
  • Entrez Gene
    HAUS6 (a.k.a. Dgt6, FAM29A)
  • Promoter polyhedrin promoter

Cloning Information for Gene/Insert 4

  • Cloning method Gibson Cloning
  • 5′ sequencing primer AAACTGGATACTATTGCAC
  • 3′ sequencing primer AAACATCAGGCATCATTAG
    • (Common Sequencing Primers)

Gene/Insert 5

  • Gene/Insert name
    HAUS7
  • Species
    H. sapiens (human); codon-optimized form for expression in Trichoplusia ni cells
  • GenBank ID
    NM_001385482.1
  • Entrez Gene
    HAUS7 (a.k.a. UCHL5IP, UIP1)
  • Promoter polyhedrin promoter

Cloning Information for Gene/Insert 5

  • Cloning method Gibson Cloning
  • 5′ sequencing primer aaacctaatgatgcctg
  • 3′ sequencing primer aaccccgattgagatatag
    • (Common Sequencing Primers)

Gene/Insert 6

  • Gene/Insert name
    HAUS4
  • Species
    H. sapiens (human); codon-optimized form for expression in Trichoplusia ni cells
  • GenBank ID
    NM_001166269.2
  • Entrez Gene
    HAUS4 (a.k.a. C14orf94, FLJ20424, FLJ40661)
  • Promoter polyhedrin promoter

Cloning Information for Gene/Insert 6

  • Cloning method Gibson Cloning
  • 5′ sequencing primer aacgctctatggtctaaag
  • 3′ sequencing primer aaacgtgcaatagtatc
    • (Common Sequencing Primers)

Gene/Insert 7

  • Gene/Insert name
    HAUS5
  • Species
    H. sapiens (human); codon-optimized form for expression in Trichoplusia ni cells
  • GenBank ID
    NM_015302.2
  • Entrez Gene
    HAUS5 (a.k.a. dgt5, KIAA0841)
  • Promoter polyhedrin promoter

Cloning Information for Gene/Insert 7

  • Cloning method Gibson Cloning
  • 5′ sequencing primer AAACTGGATACTATTGCAC
  • 3′ sequencing primer AAACATCAGGCATCATTAG
    • (Common Sequencing Primers)

Gene/Insert 8

  • Gene/Insert name
    HAUS8
  • Species
    H. sapiens (human); codon-optimized form for expression in Trichoplusia ni cells
  • GenBank ID
    NM_033417.2
  • Entrez Gene
    HAUS8 (a.k.a. DGT4, HICE1, NY-SAR-48)
  • Promoter polyhedrin promoter

Cloning Information for Gene/Insert 8

  • Cloning method Gibson Cloning
  • 5′ sequencing primer aaacctaatgatgcctg
  • 3′ sequencing primer aaccccgattgagatatag
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBIG2abc_hsHAUScomplex_FL was a gift from Thomas Surrey (Addgene plasmid # 202203 ; http://n2t.net/addgene:202203 ; RRID:Addgene_202203)

  • For your References section:

    Microtubule binding of the human augmin complex is directly controlled by importins and Ran-GTP. Ustinova K, Ruhnow F, Gili M, Surrey T. J Cell Sci. 2023 Jun 15;136(12):jcs261096. doi: 10.1242/jcs.261096. Epub 2023 Jun 26. 10.1242/jcs.261096 PubMed 37357828
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