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Addgene

Vh-HA-2xDHFR
(Plasmid #20218)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20218 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    2xDHFR
  • Alt name
    dihydrofolate reductase
  • Alt name
    fol A
  • Alt name
    ECK0049
  • Species
    E. coli
  • Insert Size (bp)
    1185
  • Entrez Gene
    folA (a.k.a. b0048, ECK0049, tmr, tmrA)
  • Tags / Fusion Proteins
    • Vh (N terminal on insert)
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

murine VH chain signal peptide followed by a HA
tag and two repeats of DHFR

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Vh-HA-2xDHFR was a gift from Carolyn Bertozzi (Addgene plasmid # 20218 ; http://n2t.net/addgene:20218 ; RRID:Addgene_20218)

  • For your References section:

    Conditional glycosylation in eukaryotic cells using a biocompatible chemical inducer of dimerization. Czlapinski JL, Schelle MW, Miller LW, Laughlin ST, Kohler JJ, Cornish VW, Bertozzi CR. J Am Chem Soc. 2008 Oct 8. 130(40):13186-7. 10.1021/ja8037728 PubMed 18788807
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