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Addgene

pL0_EF-lysA
(Plasmid #202173)

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Item Catalog # Description Quantity Price (USD)
Plasmid 202173 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pL0R
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    lysA terminator
  • Species
    Phaeodactylum tricornutum
  • Insert Size (bp)
    495
  • Mutation
    None

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pL0_EF-lysA was a gift from Christopher Dupont (Addgene plasmid # 202173 ; http://n2t.net/addgene:202173 ; RRID:Addgene_202173)

  • For your References section:

    Universal loop assembly: open, efficient and cross-kingdom DNA fabrication. Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, Bielinski V, von Dassow P, Dupont CL, Federici F. Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. Epub 2020 Feb 5. 10.1093/synbio/ysaa001 PubMed 32161816
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