pMC-cIAP1-FL
(Plasmid #20209)

• Depositing Lab: Martin Holcik
• Publication: Zhao et al Cell Death Differ. 2009 Feb . 16(2):244-52.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Modifications to backbone: The expression plasmid pMC was constructed by inserting a synthetic polylinker (5′-AGC TTG CGG CCG CTA GCC TCG AGG GAT CCT CTA GAA TGC A-3′) into a pcDNA3 vector (Invitrogen) followed by insertion of the chloramphenicol acetyl transferase (CAT) coding sequence. The CAT insert was generated by PCR amplification using pCAT as a template.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cIAP1 3'UTR
• Alt name: HIAP2, MIHB
• Species: H. sapiens (human)
• Insert Size (bp): 483
• Entrez Gene: BIRC2 (a.k.a. API1, HIAP2, Hiap-2, MIHB, RNF48, c-IAP1, cIAP1)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CAT-F (5'-GTTTGTGATGGCTTCCATGTC-3')

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The 3′UTR was generated by PCR amplification from a liver cDNA library (Invitrogen) and the expression plasmid pMC-cIAP1-FL was constructed by inserting the 3′UTR of cIAP1 downstream of CAT in the pMC construct.