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Addgene

pGEX-2T_RV-B14_VP1
(Plasmid #202074)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202074 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-2T
  • Backbone size w/o insert (bp) 4948
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    Room Temperature
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RV-B14 VP1 protein with Histag
  • Species
    Synthetic
  • Promoter tac
  • Tags / Fusion Proteins
    • GST (N terminal on backbone)
    • 6xHis-tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer M13_pUC_rev_primer
  • 3′ sequencing primer pGEX_3_primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert cloned to plasmid with BamHI (5') and EcoRI (3') restriction

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-2T_RV-B14_VP1 was a gift from Vesa Hytönen (Addgene plasmid # 202074 ; http://n2t.net/addgene:202074 ; RRID:Addgene_202074)

  • For your References section:

    Assessment of Enterovirus Antibodies during Early Childhood Using a Multiplex Immunoassay. Jouppila NVV, Lehtonen J, Seppala E, Puustinen L, Oikarinen S, Laitinen OH, Knip M, Hyoty H, Hytonen VP. Microbiol Spectr. 2023 Jun 15;11(3):e0535222. doi: 10.1128/spectrum.05352-22. Epub 2023 May 25. 10.1128/spectrum.05352-22 PubMed 37227147
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