pGEX-2T_CVA4_VP1
(Plasmid #202068)

• Purpose: Bacterial expression of VP1 protein from coxsackievirus A4 (CAV4). Contains N-terminal GST-tag and C-terminal His6-tag.
• Depositing Lab: Vesa Hytönen
• Publication: Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e0535222. doi: 10.1128/spectrum.05352-22. Epub 2023 May 25.

Backbone

• Vector backbone: pGEX-2T
• Backbone size w/o insert (bp): 4948
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: Room Temperature
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CVA4 VP1 protein with Histag
• Species: Synthetic
• Promoter: tac
• Tags / Fusion Proteins:
  • GST (N terminal on backbone)
  • 6xHis-tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: M13_pUC_rev_primer
• 3′ sequencing primer: pGEX_3_primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert cloned to plasmid with BamHI (5') and EcoRI (3') restriction