pJT365 AAV-Rapamycin Inducible-S3H
(Plasmid
#202066)
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PurposeInducible AAV activator
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 202066 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepJT360
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Vector typeMammalian Expression, AAV ; AAV with reporter
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAAV-Rapamycin Inducible-S3H
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SpeciesH. sapiens (human)
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MutationITR deletion
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
ITR deletion may be problematic for AAV . Please visit https://www.biorxiv.org/content/10.1101/2023.05.12.540558v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJT365 AAV-Rapamycin Inducible-S3H was a gift from Michael Bassik & Lacra Bintu (Addgene plasmid # 202066 ; http://n2t.net/addgene:202066 ; RRID:Addgene_202066) -
For your References section:
Development of compact transcriptional effectors using high-throughput measurements in diverse contexts. Tycko J, Van MV, A, DelRosso N, Yao D, Xu X, Ludwig C, Spees K, Liu K, Hess GT, Gu M, Mukund AX, Suzuki PH, Kamber RA, Qi LS, Bintu LB, Bassik MC.. bioRxiv. (PrePrint) 10.1101/2023.05.12.540558