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pJT315 AAV-Rapamycin Inducible-ZNF473KRAB-Citrine
(Plasmid #202064)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202064 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJT360
  • Vector type
    Mammalian Expression, AAV ; AAV with reporter

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AAV-Rapamycin Inducible-ZNF473KRAB-Citrine
  • Species
    H. sapiens (human)
  • Mutation
    ITR deletion

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

ITR deletion may be problematic for AAV . Please visit https://www.biorxiv.org/content/10.1101/2023.05.12.540558v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJT315 AAV-Rapamycin Inducible-ZNF473KRAB-Citrine was a gift from Michael Bassik & Lacra Bintu (Addgene plasmid # 202064 ; http://n2t.net/addgene:202064 ; RRID:Addgene_202064)

  • For your References section:

    Development of compact transcriptional effectors using high-throughput measurements in diverse contexts. Tycko J, Van MV, Aradhana, DelRosso N, Ye H, Yao D, Valbuena R, Vaughan-Jackson A, Xu X, Ludwig C, Spees K, Liu K, Gu M, Khare V, Mukund AX, Suzuki PH, Arana S, Zhang C, Du PP, Ornstein TS, Hess GT, Kamber RA, Qi LS, Khalil AS, Bintu L, Bassik MC. Nat Biotechnol. 2024 Nov 1. doi: 10.1038/s41587-024-02442-6. 10.1038/s41587-024-02442-6 PubMed 39487265
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