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1BR9-AtWUSSTOP-Δα3
(Plasmid #202056)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202056 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    1BR9
  • Backbone size w/o insert (bp) 5513
  • Total vector size (bp) 6336
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    AtWUS E. Coli Codon Optimized, third helix of homeodomain deleted
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    810
  • Mutation
    third helix of homeodomain deleted (AA82-102)
  • GenBank ID
    NM_127349
  • Entrez Gene
    WUS (a.k.a. AT2G17950, PGA6, T27K22.18, T27K22_18, WUS1, WUSCHEL, WUSCHEL 1)
  • Promoter T7 Promoter
  • Tags / Fusion Proteins
    • 6xHis (N terminal on backbone)
    • GFP11 (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer TAATACGACTCACTATAGGG
  • 3′ sequencing primer GCTAGTTATTGCTCAGCGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    1BR9-AtWUSSTOP-Δα3 was a gift from Markita Landry (Addgene plasmid # 202056 ; http://n2t.net/addgene:202056 ; RRID:Addgene_202056)

  • For your References section:

    Delivered complementation in planta (DCIP) enables measurement of peptide-mediated protein delivery efficiency in plants. Wang JW, Squire HJ, Goh NS, Ni HM, Lien E, Wong C, Gonzalez-Grandio E, Landry MP. Commun Biol. 2023 Aug 12;6(1):840. doi: 10.1038/s42003-023-05191-5. 10.1038/s42003-023-05191-5 PubMed 37573467
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