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Addgene

pT/CMV-SV40-LgT
(Plasmid #20204)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20204 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    colE1/ampR
  • Backbone manufacturer
    Ohlfest Lab
  • Backbone size w/o insert (bp) 4663
  • Vector type
    Transposon

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5-alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SV40LgT
  • Species
    M. musculus (mouse); viral
  • Insert Size (bp)
    2473

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT/CMV-SV40-LgT was a gift from John Ohlfest (Addgene plasmid # 20204 ; http://n2t.net/addgene:20204 ; RRID:Addgene_20204)

  • For your References section:

    De novo induction of genetically engineered brain tumors in mice using plasmid DNA. Wiesner SM, Decker SA, Larson JD, Ericson K, Forster C, Gallardo JL, Long C, Demorest ZL, Zamora EA, Low WC, SantaCruz K, Largaespada DA, Ohlfest JR. Cancer Res. 2009 Jan 15. 69(2):431-9. 10.1158/0008-5472.CAN-08-1800 PubMed 19147555
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