pCitro-15a
(Plasmid
#202033)
-
Purposep15a-based plasmid that encodes a conjugative system synthesized from metagenomic data for conjugation to Citrobacter
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 202033 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepACYCDuet-1
- Total vector size (bp) 57346
-
Modifications to backboneIntroduction of yeast replication and selection elements CEN/ARS/HIS3
-
Vector typeSynthetic Biology
-
Selectable markersHIS3
Growth in Bacteria
-
Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameconjugative system from a Citrobacter spp.
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCitro-15a was a gift from David Edgell (Addgene plasmid # 202033 ; http://n2t.net/addgene:202033 ; RRID:Addgene_202033) -
For your References section:
De Novo Synthesis of a Conjugative System from Human Gut Metagenomic Data for Targeted Delivery of Cas9 Antimicrobials. Hamilton TA, Joris BR, Shrestha A, Browne TS, Rodrigue S, Karas BJ, Gloor GB, Edgell DR. ACS Synth Biol. 2023 Dec 15;12(12):3578-3590. doi: 10.1021/acssynbio.3c00319. Epub 2023 Dec 4. 10.1021/acssynbio.3c00319 PubMed 38049144
