pFastBac1 Flag Su(Z)2
(Plasmid #20202)

• Depositing Lab: Nicole Francis
• Publication: Lo et al Mol Cell Biol. 2009 Jan . 29(2):515-25.

Backbone

• Vector backbone: pFastBac1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4775
• Vector type: Insect Expression
• Selectable markers: Gentamicin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Su(Z)2
• Alt name: Suppressor 2 of Zeste
• Alt name: Arp
• Alt name: CG3905
• Species: D. melanogaster (fly)
• Insert Size (bp): 6201
• Entrez Gene: Su(z)2 (a.k.a. Dmel_CG3905, Arp, CG3905, Dmel\CG3905, SU(Z)2, SUZ2, Su(z)2D, Su(z)3, Su(z)[[2]], Suz2, l(2)k06344, su(z)2, su(z)3)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BssHII (not destroyed)
• 3′ cloning site: SpeI (not destroyed)
• 5′ sequencing primer: Polyhedrin forward
• 3′ sequencing primer: pFastBac reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Su(Z)2 cDNA came from P. Adler via Mol Cell Biol (1994), 14(3):1721-32.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry