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Flag-GFP-TurboID-NES_pLX304
(Plasmid #202019)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202019 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLX304
  • Total vector size (bp) 9470
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Flag-GFP-TurboID-NES
  • Species
    Synthetic
  • Insert Size (bp)
    1761
  • Promoter CMV
  • Tags / Fusion Proteins
    • Flag (N terminal on insert)
    • GFP (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag-GFP-TurboID-NES_pLX304 was a gift from Alice Ting (Addgene plasmid # 202019 ; http://n2t.net/addgene:202019 ; RRID:Addgene_202019)

  • For your References section:

    Dynamic mapping of proteome trafficking within and between living cells by TransitID. Qin W, Cheah JS, Xu C, Messing J, Freibaum BD, Boeynaems S, Taylor JP, Udeshi ND, Carr SA, Ting AY. Cell. 2023 Jun 23:S0092-8674(23)00596-2. doi: 10.1016/j.cell.2023.05.044. 10.1016/j.cell.2023.05.044 PubMed 37385249
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