pMT1-24X-NOG(AtUBI10)
(Plasmid #202016)

• Purpose: T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the AtUBI10 promoter.
• Depositing Lab: Michael Smanski
• Publication: Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue 13, 21 July 2023, Pages 7083–7093

Backbone

• Vector backbone: pTRANS_220d
• Backbone size w/o insert (bp): 8729
• Total vector size (bp): 19505
• Vector type: Plant Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: dCas9-24XGP41
• Species: Synthetic; Streptococcus pyogenes
• Promoter: AtUBI10

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: AarI (destroyed during cloning)
• 3′ cloning site: AarI (destroyed during cloning)
• 5′ sequencing primer: none

Gene/Insert 2

• Gene/Insert name: NbGP41P-sfGFP-VP64-GB1
• Species: Synthetic; Lama glama, herpes simplex virus
• Promoter: AtUBI10
• Tags / Fusion Proteins:
  • sfGFP
  • GB1

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: AarI (destroyed during cloning)
• 3′ cloning site: AarI (destroyed during cloning)
• 5′ sequencing primer: none

Resource Information

• Supplemental Documents:
  • pmt1-24x-nog(atubi10).gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry