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Addgene

pEF1a-PEmax-P2A-GFP
(Plasmid #202000)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202000 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-PEmax-P2A-GFP
  • Total vector size (bp) 11128
  • Vector type
    Mammalian Expression, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PEmax-P2A-GFP
  • Species
    Synthetic; SpCas9 is from Streptococcus pyogenes
  • Insert Size (bp)
    7143
  • GenBank ID
    NA
  • Promoter EF1a
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer cgatgtacgggccagatataggctccggtgcccgtcag
  • 3′ sequencing primer ctatagtgagtcgtattagctcacgacacctgaaatggaagaaaaaaactttgaacc
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF1a-PEmax-P2A-GFP was a gift from Daniel Geschwind (Addgene plasmid # 202000 ; http://n2t.net/addgene:202000 ; RRID:Addgene_202000)
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