CROP-seq-opti-dsRed
(Plasmid
#201999)
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Purpose(Empty Backbone) Based on CROP-seq-opti (addgene #106280), we replaced the PURO resistance gene with a dsRed.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 201999 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneCROP-seq-opti
- Backbone size (bp) 10293
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Vector typeMammalian Expression, Lentiviral, CRISPR
- Promoter EF1a
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Tag
/ Fusion Protein
- dsRed (N terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer TTTCCCATGATTCCTTCATATTTGC
- 3′ sequencing primer CTGTTTCCAGCATAGCTCTTAAAC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CROP-seq-opti-dsRed was a gift from Daniel Geschwind (Addgene plasmid # 201999 ; http://n2t.net/addgene:201999 ; RRID:Addgene_201999)
Map uploaded by the depositor.
