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pcDNA3.1-KIT-V5/HIS
(Plasmid #201986)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201986 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1-V5/His
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Extend the time for phenotypic expression for an extra one hour before plating the bacteria
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KIT receptor tyrosine kinase
  • Species
    H. sapiens (human)
  • Entrez Gene
    KIT (a.k.a. C-Kit, CD117, MASTC, PBT, SCFR)
  • Promoter CMV
  • Tag / Fusion Protein
    • V5, 6xHis (C terminal on backbone)

Cloning Information

  • Cloning method TOPO Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-KIT-V5/HIS was a gift from Pavel Krejčí (Addgene plasmid # 201986 ; http://n2t.net/addgene:201986 ; RRID:Addgene_201986)

  • For your References section:

    One reporter for in-cell activity profiling of majority of protein kinase oncogenes. Gudernova I, Foldynova-Trantirkova S, Ghannamova BE, Fafilek B, Varecha M, Balek L, Hruba E, Jonatova L, Jelinkova I, Kunova Bosakova M, Trantirek L, Mayer J, Krejci P. Elife. 2017 Feb 15;6. pii: e21536. doi: 10.7554/eLife.21536. 10.7554/eLife.21536 PubMed 28199182
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