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Addgene

pcDNA3.1-INSRR-V5/HIS
(Plasmid #201981)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201981 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1-V5/His
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    INSRR receptor tyrosine kinase
  • Species
    H. sapiens (human)
  • Entrez Gene
    INSRR (a.k.a. IRR)
  • Promoter CMV
  • Tag / Fusion Protein
    • V5, 6xHis (C terminal on backbone)

Cloning Information

  • Cloning method TOPO Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS result found P14H mutation in INSRR receptor tyrosine kinase, but it does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-INSRR-V5/HIS was a gift from Pavel Krejčí (Addgene plasmid # 201981 ; http://n2t.net/addgene:201981 ; RRID:Addgene_201981)

  • For your References section:

    One reporter for in-cell activity profiling of majority of protein kinase oncogenes. Gudernova I, Foldynova-Trantirkova S, Ghannamova BE, Fafilek B, Varecha M, Balek L, Hruba E, Jonatova L, Jelinkova I, Kunova Bosakova M, Trantirek L, Mayer J, Krejci P. Elife. 2017 Feb 15;6. pii: e21536. doi: 10.7554/eLife.21536. 10.7554/eLife.21536 PubMed 28199182
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