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Addgene

TRAPa(E162A,M164A,R167A)-3xFLAG-pCDNA
(Plasmid #201964)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201964 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3.1(+)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SSR1
  • Species
    H. sapiens (human)
  • Mutation
    E162A,M164A,R167A
  • Entrez Gene
    SSR1 (a.k.a. TRAPA)
  • Tag / Fusion Protein
    • 3xFLAG-tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TRAPa(E162A,M164A,R167A)-3xFLAG-pCDNA was a gift from Ville Paavilainen (Addgene plasmid # 201964 ; http://n2t.net/addgene:201964 ; RRID:Addgene_201964)

  • For your References section:

    Molecular view of ER membrane remodeling by the Sec61/TRAP translocon. Karki S, Javanainen M, Rehan S, Tranter D, Kellosalo J, Huiskonen JT, Happonen L, Paavilainen V. EMBO Rep. 2023 Dec 6;24(12):e57910. doi: 10.15252/embr.202357910. Epub 2023 Nov 20. 10.15252/embr.202357910 PubMed 37983950
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