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Addgene

lenti.DTR.GFP
(Plasmid #201962)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201962 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    lenti.Cas9.BFP.Blast, #196714
  • Backbone size w/o insert (bp) 5738
  • Total vector size (bp) 7099
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    EGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Diptheria Toxin Receptor
  • Alt name
    DTR, HBEGF
  • Species
    Chlorocebus sabaeus
  • Insert Size (bp)
    1362
  • GenBank ID
    XM_008014669.2
  • Promoter EF1alpha
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsiWI (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer tctagaactagtggatc
  • 3′ sequencing primer GATACCGTCGACCTCGA
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Insert was PCR amplified from pAAV-FLEX-DTR-GFP (Addgene #124364, a gift from Eiman Azim & Thomas Jessell)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    lenti.DTR.GFP was a gift from Bernhard Schmierer (Addgene plasmid # 201962 ; http://n2t.net/addgene:201962 ; RRID:Addgene_201962)

  • For your References section:

    An idiosyncratic zonated stroma encapsulates desmoplastic liver metastases and originates from injured liver. Fernandez Moro C, Geyer N, Harrizi S, Hamidi Y, Soderqvist S, Kuznyecov D, Tidholm Qvist E, Salmonson Schaad M, Hermann L, Lindberg A, Heuchel RL, Martin-Bernabe A, Dhanjal S, Navis AC, Villard C, Del Valle AC, Bozoky L, Sparrelid E, Dirix L, Strell C, Ostman A, Schmierer B, Vermeulen PB, Engstrand J, Bozoky B, Gerling M. Nat Commun. 2023 Aug 18;14(1):5024. doi: 10.1038/s41467-023-40688-x. 10.1038/s41467-023-40688-x PubMed 37596278
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